Coding

Part:BBa_K4348003:Design

Designed by: Jonathan Cheng   Group: iGEM22_McGill   (2022-09-16)


E. copro unknown MFS transporter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1063
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 847
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 233
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimized for expression in B. subtilis


Source

Eubacterium coprostanoligenes

References